２０１９年２月１５日 Prof. Michael Elbaum

日時：２０１９年２月１５日（金）　１１：００〜１２：３０
場所：首都大学東京　８号館３０２号室
講師：Prof. Michael Elbaum (Weizmann Institute of Science)
題目：Cellular imaging in 3D with cryo-STEM tomography

要旨：Cryo-electron microscopy has achieved unprecedented resolution in recent years with new technological breakthroughs. However its practice is traditionally restricted to the study of ultra-thin specimens. While tomography introduced a view in three dimensions, the thickness limitations of traditional imaging modalities remain. We have introduced the method of scanning transmission electron microscopy (STEM) for cryo-tomography of biological specimens. STEM is inherently suited to thicker samples, in some cases reaching intact cells. Moreover, the image contrast based on electron scattering can be interpreted quantitatively. The talk will describe the methodology and a number of applications to intracellular crystallization and self-assembly.